Peace of mind in One Solution


 "A Liquid Oxygen-Based Disinfectant"


The Next Generation in Oxidation Technology

Liquid Oxygen-Based water treatment  

Conventional treatment protocols for municipal water and waste water treatment are not able to eliminate biologic growth in filtration systems, on ultraviolet systems, nor within distribution systems. Chemical applications are not able to address the biologic and chemical contaminants in many industrial waste streams. The technology oxidation has not advanced in more than thirty years. SAFE-ZONE ROS has brought aqueous reactive oxygen species into the 21st century by mimicking reactive oxygen species organically in a safer, user friendly formula. "A Reactive Oxygen Species."  

The Product Idea: SAFE-ZONE ROS 

The "Reactive Oxygen Species that is NSF APPROVED" has improved the technology of disinfection and oxidation by mimicking nature. Nature utilizes a variety of “reactive oxygen species” (ROS) and utilizing specific minerals and metals to aid in the distribution of ROS and act as catalysts for ROS reactions against microbes and organic contaminants.

.SafeZone ROS advances water treatment technology by translating what nature does into a simple to use liquid formulation. The result is a higher reactive oxygen range, for the management of viruses, bacteria, molds and bio-film that is more than twelve times that of conventional chlorine treatments, it far exceeds peroxides and it provides a superior residual protection with reactive oxygen species.

An Environmentally Safe & Effective Alternative

• More than 12 time the re-activity of chlorine

• Delivers oxidation energy controlled and measured by ORP

• Free Oxygen radical generation

• Controls pathogens/microbes on contact

• Detoxifies organics

• Residual is Non-Toxic: Oxygen and mineral monoxides

• By-products are Non-Toxic

• 21st Century hybrid of an aqueous reactive oxygen species that comes in a bottle.

In comparative lab and field Tests, Safe Zone ROS out-perform:

Peroxide               Peroxide + Sani-T-10      Peracetic acid                Storox

HDH Peroxy         Jet Oxide                         Chemchlor                     ProOxine

Sani-T-10             Quat SL                            Acidize DS-50               Dowicide-1

Ethyl Alcohol        Isopropyl Alcohol              Calcium Hypochlorite    Sodium Hypochlorite

Glutaraldehyde     Formaldehyde                  Tsunami 100

Commercial Applications                        Industrial Applications

Institutional cleaning                                 Waste Water Treatment

Institutional disinfection                             Bacteria Free Ice

Potable Water Treatment

Water Disinfection                                     Odor Control

Odor Control                                              BDO/COD control

Algae Control                                             VOC/TOC control

Bio-film control                                           Pathogen control

Well flushing                                              Scale Control

Post Harvesting Decontamination             Precipitation of metals

Foliage and irrigation application              Oxidation of Cyanide  

One of the most critical concerns in today's world, is the control of organic contaminants, and pathogen populations, in our water supply, distribution systems and waste streams. Using a reactive oxygen species is one of the most effective methods of protecting against pathogen contamination in the environment. Reactive oxygen species are a natural unstable form of free radicals oxygen (O3 rather than O2). A reactive oxygen species releases the extra oxygen atom, in the form of a "free radical". These free radicals act somewhat like an unguided missile; hitting the organic target (contaminant, or pathogen), and destroying the target. The free radical function is very different from that usually found in the use of such products like chlorine. Reactive oxygen species does not poison, it actually destroys the target. The "target" cannot become immune to this free radical action.

The use of "mechanically generated” has existed since 1860’s, for the control of pathogens in potable water systems. For over one hundred and thirty years we have attempted to prefect an inexpensive method of generating reactive oxygen species, by mechanical means. Unfortunately, the use of reactive oxygen species still remains an expensive capital investment for the average user in need of environmental control and improvement.

SAFE-ZONE ROS , was developed to act as a liquid form of free radical oxygen; just as reactive oxygen species generates free radical oxygen. SAFE-ZONE ROS ,requires no expensive capital investment; only a simple injection into the water stream to be treated; or SAFE-ZONE ROS , may be sprayed onto hard surfaces, no rinse needed, no harmful residue & nontoxic. By applying Safe Zone to the water enables the user to control cross contamination and the spread of pathogens, and/or organics in water or on surfaces (bacterial slime, algae, fungus, etc.) and eliminating the bio-film where the pathogens are embedded leaving a completely clean surface. All this performance at drinking water standards.

The only residual after the application of SAFE-ZONE ROS , is in the water, or on surfaces, is atomic oxygen and GRAS mineral oxides. No chemicals, or harmful residuals are left to be a concern.

SAFE-ZONE ROS , is a unique mixture of minerals and oxygen in liquid form. When SAFE-ZONE ROS comes in contact with pathogens or organic contaminants, it releases nascent oxygen. This “release as needed” property of SAFE-ZONE ROS , offers a strong downstream residual. Also can be frozen and as it melts continues to control cross contamination and eliminate bio-film even after it melts.

What is Nascent Oxygen?

Oxygen, as it occurs naturally, is a compound of two atoms of oxygen. Nascent Oxygen refers to making available a single atom of oxygen.

Why Nascent Oxygen?

It provides the highest efficiency of oxidation. Oxidation is an aggressive reaction that dramatically changes the physical properties of a target compound.

Of the many elements that will cause oxidation reactions, nascent oxygen is the most aggressive agent and gives the added bonus of not producing toxic byproducts.

For pathogen control, the amount of nascent oxygen necessary to eliminate pathogens is much less than other oxidants or disinfectants.

Salmonella enteric 10708 bench test

Solution              PPM            CFU/ml: Time Zero       CFU/ml: 30 min         Log Reduction

Chlorine                10                2.00 E +07                    1.89 E +05                   2.02

Chlorine               100               1.58 E +07                          < 5                          6.49

Safe Zone ROS     10                2.00 E +07                          < 5                          6.69

Penicillium digitatum bench test

Concentrations (mg/L) of sanitizer that inhibit 50% (EC50) or 99% (EC50) of germination of conidia of P. digitatum exposed to them for 10 min at 23 C. Germination was determined after incubation on potato dextrose agar for 18H. Concentrations and 95% confidence intervals (Cl) were estimated using Finney’s Probit

Chemical                  EC50                 95% CL              EC50                   95% CL

Glutaraldehyde         1243                1116-1372           2774                  2503-3157

Formaldehyde           1200               1023-1392            2502                 2237-3140

Peroxide                      676                  610-745              1691                 1537-1893

Chlorine                      44.3                 31.1-60.3             116.6                 91.1-173.7

Safe Zone ROS            9.4                  4.9-16.5               25.3                   17.7-56.

"Dr Trevor Suslow of University of California @ Davis studies demonstrate that @ 670 ORP

Safe Zone ROS shows negative detect of Salmonella, Listeria and E-Coli in 10 seconds or less."

Ballast Treatment with SAFE-ZONE ROS AG 5000 

Produce Wash

Are you currently using chlorine, chlorine dioxide, gaseous reactive oxygen species, peracetic acid, menadione/vitamin K (SeaKleen), Uv or a combination of these chemicals to disinfect your ballast water prior to discharge? 

Would you like a system that eliminates like reactive oxygen species and generates water and oxygen molecules as by-products while at the same time ELIMINATE THE BIO-FILM where the bacteria, mold and viruses are embedded. If you have a reactive oxygen species generator on-board would you like to minimize another shipboard hazard (pure oxygen, reactive oxygen species gas scrubber and etc)?

GreenAgri has developed a new disinfectant, AG 5000, as an alternative to chlorine, chlorine dioxide, gaseous reactive oxygen species. AG 5000 is a mineral oxy-chloride ion that reacts in water to generate reactive oxygen species (ROS) (ie. Peroxides, Singlet oxygen, superanion, and hydroxyl ions). ROS molecules react with any microorganisms killing them immediate. Bacteria, viruses, fungi and related biofilms do not develop a tolerance to ROS. AG 5000, is a broad based oxidant that is NSF 60 approved for use in potable water treatment systems up to 100 mg/l. We use ORP to control our dosage and feed rate. In a study using ORP to measure the oxidant potential, the following data was collected:

                              ORP Level             CFU/100ml

                                +200 mV                300        

                                +300 mV                   36 

                                +400 mV                    3   

                                +600 mV                    ND   

Systems using chemical biocides need to be designed to avoid discharging unwanted concentrations of residual biocide. In addition another issue with systems using chemical biocides is the potential for the production of disinfection byproducts, such as trihalomethanes and halo-acetic acids. The disinfectant must meet G8 and G9 standards of verifying a method that translates the disinfection level.

Discharge limits for Ballast Water Discharge

  Agency              Vibrio Cholera                     E.Coli                          Intestinal Enterococci

IMO BW               <1 CFU/100 ml                     250 CFU/100 ml            100 CFU/100 ml

US EPA                         same                           126 CFU/100 ml            <33 CFU/100 ml

In a study conducted in California, using a fresh water sample taken from the Delta Bay (Northern California-Sacramento River), we found the microorganism levels (mpn/100 ml) were: 2500+ CFU/100 ml, and E.coli was >4+. Using 10 mg/1 of Safe Zone AG 5000, we were able to reduce the microbiological count (mpn) to: ND/100 ml and non-detect on E.coli in less than 20 minutes of contact time. We also observed an overall improvement in the treatment system. Raw water turbidity was 5.0 NTU and in the settling zone before filtration (using Safe Zone AG 5000) the turbidity was 0.125 NTU. Before the plant trial, the average turbidity in the settled water was 1.3 NTU. The operator wanted to maintain a free chlorine residual of 0.8 mg/l to 1.2 mg/l. He found that on initial startup, the free chlorine residual dropped slightly because of the removal of the biofilm in the system but returned after several hours. The net results was an overall improved treatment program with non-detect microorganism and a very low free chlorine residual in the ballast water.

Contact GreenAgri Solutions, LLC. at (352) 610-4808 or email:

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